Understanding the effects of process parameters in the bioscouring of cotton and their interactions on pectate lyase activity by factorial design analysis

Pectinases can be used to remove impurities and hydrophobic material from cotton fibers by the degradation of pectic substances. The biochemical characterization evaluation process parameters that influence enzyme’s activity are mandatory optimize industrial application. In present study, a factorial experimental design was conducted evaluate commercial pectate lyase at different reaction conditions temperature, pH, nonionic surfactant concentration, aiming its application in bioscouring textiles. main effects all three factors were found statistically significant. second-order interactions between temperature pH also important. Maximum on polygalacturonic acid achieved 60 °C, 9, without surfactant. These results provided support for subsequent assays, resulting high level cleanliness good wettability knitted fabric lowest enzyme concentration (1 g/l), could make textile processing more sustainable.